Journal: Brazilian Journal of Physical Therapy

Article Title: Can transcutaneous electrical nerve stimulation improve achilles tendon healing in rats?

doi: 10.1590/bjpt-rbf.2014.0107

Figure Lengend Snippet: Comparison of the quantity of collagen types III (%) in the tendon tissue between the TENS and Simulation groups. Data expressed in mean and standard deviation.

Article Snippet: The expression of collagen I and III, observed in the immunohistochemical reaction with the polyclonal antibodies for the collagen types I and III (Affinity Purified Anti-Collagen Type I and Type III, Rabbit Anti-Human, Rockland Inc., Gilbertsville, PA, USA), was analyzed by quantifying the area corresponding to each type of collagen.

Techniques: Comparison, Standard Deviation

Journal: Brazilian Journal of Physical Therapy

Article Title: Can transcutaneous electrical nerve stimulation improve achilles tendon healing in rats?

doi: 10.1590/bjpt-rbf.2014.0107

Figure Lengend Snippet: Measures of variables related to group, assessment, and results of Analysis of Variance (ANOVA).

Article Snippet: The expression of collagen I and III, observed in the immunohistochemical reaction with the polyclonal antibodies for the collagen types I and III (Affinity Purified Anti-Collagen Type I and Type III, Rabbit Anti-Human, Rockland Inc., Gilbertsville, PA, USA), was analyzed by quantifying the area corresponding to each type of collagen.

Techniques: Standard Deviation

Journal: Inflammation and Regeneration

Article Title: Novel artificial nerve transplantation of human iPSC-derived neurite bundles enhanced nerve regeneration after peripheral nerve injury

doi: 10.1186/s41232-024-00319-4

Figure Lengend Snippet: Early-stage tissue evaluation after neurite bundle-derived artificial nerve transplantation. a , b Immunohistochemistry images with anti-NFH and anti-CD31 antibodies. Dotted arrows indicate the proximal side of the intact nerve areas, and solid arrows indicate the NFH- and CD31-positive regeneration tip in the reconstructed tissue. Scale bars = 1 mm. c , d Quantitative comparison of axonal extension distance was indicated by NFH-positive areas and that of vascular elongation distance was demonstrated by CD31-positive areas at 1 and 2 weeks after transplantation. Significant differences between groups elongation distances were observed in 2-week samples only. e , f Iba1 immunohistochemistry image of the proximal part of the regenerated tissue at 2 weeks after transplantation (e). Scale bars = 1 mm. Quantitative analysis of Iba1-positive areas demonstrated that a larger number of macrophages aggregated in the Auto, motor TP and sensory TP (f). ( n = 3). * p < 0.05, ** p < 0.01, N.S. = not significant. Data are represented as the mean ± SEM

Article Snippet: The primary antibodies used in this study were human polyclonal anti-pan-ELAVL (ELAV-like protein 2/3/4) antibody (1:2000, kindly provided from Prof. Robert Darnell, Rockefeller University), rabbit polyclonal anti-TrkA antibody (1:500, kindly provided from Prof. Louis F. Reichardt, UCSF), chicken polyclonal anti-TrkB antibody (1:500, kindly provided from Prof. Louis F. Reichardt, UCSF), goat polyclonal anti-TrkC antibody (AF373, 1:200, R&D systems), rabbit polyclonal anti-parvalbumin antibody (PV-28, 1:1000, Swant), rabbit polyclonal anti-CGRP antibody (BML-CA1134, 1:500, Enzo Life Sciences), Goat polyclonal anti-Choline acetyltransferase antibody (AB144P, 1:200, Chemicon), Mouse monoclonal anti-Islet1 and Islet2 antibody (39.4D5, 1:200, DSHB), rabbit polyclonal anti-Neurofilament heavy polypeptide antibody (ab8135, 1:500, Abcam), goat polyclonal anti-mouse and anti-rat CD31 (AF3628, 1:100, R&D), rabbit polyclonal anti-Iba1 antibody (GtX100042, 1:500, GeneTex), goat anti-GFP antibody (600–101-215, 1:1000, Rockland).

Techniques: Derivative Assay, Transplantation Assay, Immunohistochemistry, Comparison

Journal: Hypertension

Article Title: Calpain-1 Regulation of Matrix Metalloproteinase 2 Activity in Vascular Smooth Muscle Cells Facilitates Age-Associated Aortic Wall Calcification and Fibrosis

doi: 10.1161/hypertensionaha.112.196840

Figure Lengend Snippet: Figure 3. Overexpression of calpain-1 induces collagen type I, II, and III and reduces elastin expression levels in vascular smooth muscle cells (VSMC). A, Representative Western blots of collagen I and collagen III in VSMC after infection by GFP or CANP1 adenoviruses for 48 hours. B, Photomicrographs (×400) of collagen II protein staining (brown color) from young (8-month) and old (30-month) rat aortic walls. Nuclei are counterstained with hematoxylin (blue color). L indicates lumen; M, media. C and D, Representative Western blots and average protein levels of collagen II (C) and elastin (D) in young VSMC infected with GFP or CANP1 for 48 hours. *P<0.05 compared with GFP-infected 8-month VSMC, n=3.

Article Snippet: Primary antibodies used for immunostaining (IS) and Western blotting (WB) Antibody Species Titer for IS Titer for WB Source -actin Mouse 1:10000 Sigma-Aldrich Co. LLC, St. Louis, MO calpain-1 Rabbit 1:100 1:2500 Sigma-Aldrich Co. LLC, St. Louis, MO collagen I Rabbit 1:1000 Rockland Antibodies & Assays, Gilbertsville, PA collagen II Rabbit 1:50 1:1000 Abcam, Cambridge, MA collagen III Rabbit 1:1000 Rockland Antibodies & Assays, Gilbertsville, PA elastin Rabbit 1:1000 Millipore, Billerica, MA MMP2 Mouse 1:50 1:1000 Millipore, Billerica, MA MT1MMP Rabbit 1:1000 Millipore, Billerica, MA osteonectin Rabbit 1:1000 Millipore, Billerica, MA osteoponin Rabbit 1:1000 Millipore, Billerica, MA Smad 2/3 Mouse 1:1000 Cell Signaling Technology, Inc, Danvers, MA TGF-1 Rabbit 1:500 Santa Cruz Biotechnology, Inc, Santa Cruz, CA TIMP2 Goat 1:500 R&D Systems, Inc. Minneapolis, MN 9 Figure S1: Interactions between calpain-1 and MMP2.

Techniques: Over Expression, Expressing, Western Blot, Infection, Staining

Journal: Hypertension

Article Title: Calpain-1 Regulation of Matrix Metalloproteinase 2 Activity in Vascular Smooth Muscle Cells Facilitates Age-Associated Aortic Wall Calcification and Fibrosis

doi: 10.1161/hypertensionaha.112.196840

Figure Lengend Snippet: Figure 6. Overexpression of calpain-1 regulates extracellular matrix remodeling, particularly fibrosis and vascular calcification in cultured carotid artery rings. Representative Western blot of tissue inhibitor of metalloprotease 2 (TIMP2), calpain-1, elastin, transforming growth factor-β1 (TGFβ1), (A) Smad 2/3, collagen I, II, III, (B) and osteopontin (OPN) and osteonectin (ON) (C) in cultured carotid artery rings isolated from 8-month-old rat after infection by GFP or CANP1 or TIMP2 adenoviruses for 48 hours.

Article Snippet: Primary antibodies used for immunostaining (IS) and Western blotting (WB) Antibody Species Titer for IS Titer for WB Source -actin Mouse 1:10000 Sigma-Aldrich Co. LLC, St. Louis, MO calpain-1 Rabbit 1:100 1:2500 Sigma-Aldrich Co. LLC, St. Louis, MO collagen I Rabbit 1:1000 Rockland Antibodies & Assays, Gilbertsville, PA collagen II Rabbit 1:50 1:1000 Abcam, Cambridge, MA collagen III Rabbit 1:1000 Rockland Antibodies & Assays, Gilbertsville, PA elastin Rabbit 1:1000 Millipore, Billerica, MA MMP2 Mouse 1:50 1:1000 Millipore, Billerica, MA MT1MMP Rabbit 1:1000 Millipore, Billerica, MA osteonectin Rabbit 1:1000 Millipore, Billerica, MA osteoponin Rabbit 1:1000 Millipore, Billerica, MA Smad 2/3 Mouse 1:1000 Cell Signaling Technology, Inc, Danvers, MA TGF-1 Rabbit 1:500 Santa Cruz Biotechnology, Inc, Santa Cruz, CA TIMP2 Goat 1:500 R&D Systems, Inc. Minneapolis, MN 9 Figure S1: Interactions between calpain-1 and MMP2.

Techniques: Over Expression, Cell Culture, Western Blot, Isolation, Infection